骨保护素在犬恒牙胚发育中表达及意义.docVIP

骨保护素在犬恒牙胚发育中表达及意义[摘要]目的：探讨犬乳恒牙替换过程中骨保护素在犬恒牙胚发育中的表达及意义。方法：分别对乳牙期、替牙期、恒牙期本地犬取牙胚及其周围组织,制备石蜡切片,进行HE染色和牙胚内OPG的免疫组化染色,用彩色病理图像分析系统分析各组染色强度灰度值。结果：乳牙期、替牙期犬牙胚的成牙本质细胞OPG的免疫组化强度明显高于另外一组,成釉细胞中也有阳性信号。结论：OPG可能参与恒牙胚的形成和发育。 [关键词]犬恒牙胚；骨保护素；成牙本质细胞；成釉细胞 [中图分类号]R781 [文献标识码]A [文章编号]1008-6455（2008）06-0877-2 The expression and significance of osteoprotegerin in the the development of of dogs’ permanent teeth germs CHEN Li-ping1，WU Wei1，BAI Hui-ying1，YANG Fu-sheng2 (1.Department of Dentistry, Qinghai Provincial Corps Hospital,Chinese Pepole′s Armed Police Force, Xi′ning 810001,Qinghai,China;2.College of Stomatology,Fourth Military Medical University) Abstract:ObjectiveTo study the expression and significance of osteoprotegerin（OPG）in the development of dogs’permanent teeth germs. MethodsDogs of different ages were selected as objects,selected permanent teeth germs and their peripheral tissue of dogs in different periods of primary dentition roots stability，primary dentition roots resorption,permanent dentition,preparating of paraffin slice.HE dyeing and OPG immunohistochemical dyeing were analysing the gray value of dyeing tense.ResultsOPG dyeing tense of immunohistochemic in odontoblasts of the germs in period of primary dentition roots stability and primary dentition roots resorption is stronger than the other team.OPG position signals were observed on ameloblasts. ConclusionOPG probably takes partin the formation and development of dogs’permanent teeth germs. Key words:dogs’permanent teeth germs;osteoprotegerin(OPG);odontoblasts;ameloblasts 骨保护素(osteoprotegerin，OPG)是肿瘤坏死因子受体超家族成员，其主要生物学作用是抑制破骨细胞(osteoclast,OC)形成、分化和增加骨密度，是抑制骨吸收的关键因子。有研究表明， OPG基因表达在牙滤泡，在牙齿萌出中发挥一定作用。而在恒牙胚发育中的作用却未见相关报道。本实验主要通过对犬恒牙胚成牙本质细胞中OPG的表达进行研究,探讨OPG在此过程中的作用。 1材料和方法 1.1材料及分组：动物选用本地犬共3只，分为3组，A组：乳牙牙根稳定犬(8周龄)；B组：乳牙牙根吸收犬(18周龄)；C组：成年恒牙列犬(22周龄)。 1.2试剂：兔抗人OPG多克隆抗体(北京博奥森生物工程有限公司)；SABC免疫组化检测试剂盒(北京博奥森生物工程有限公司)；DAB显色试剂盒(北京博奥森生物工程有限公司)。 1.3切片标本制作及观察：分别将实验犬以速眠新Ⅱ注射液按0.2ml/kg肌注麻醉，解剖分离双侧颈动脉后断头处死，颈动脉插