基于混合抗体的酶联免疫分析方法同时检测孔雀石绿和隐孔雀石绿.PDF

See discussions, stats, and author profiles for this publication at: /publication/305535872 Simultaneous detection of malachite green and leucomalachite green based on hybrid antibody ELISA analysis method Article · ecember 2015 OI: 10.13982/j.mfst.1673-9078.2015.12.049 CITATIONS REA S 0 48 9 authors, including: Zhen-Lin Xu Hongtao Lei South China Agricultural University South China Agricultural University 103 PUBLICATIONS 803 CITATIONS 117 PUBLICATIONS 9 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Inovation rapid detection technique and molecular recognition michanism for chemical contaminants in food View project Production of antibodies against pesticides and development of immunoasssays View project All content following this page was uploaded by Hongtao Lei on 18 September 2016. The user has requested enhancement of the downloaded file. 现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.12 基于混合抗体的酶联免疫分析方法同时检测 孔雀石绿和隐孔雀石绿 谢焕龙，王宇，徐振林，杨金易，肖治理，王弘，雷红涛，孙远明，沈玉栋 （华南农业大学食品学院，广东省食品质量安全重点实验室，广东广州 510642） 摘要：孔雀石绿是“三致”物质，常被非法使用于渔业生产。水产品和环境中常以孔雀石绿和隐孔雀石绿两种形式同时存在， 均具有强致癌性。但由于二者结构差异较大，现有的单克隆抗体只能分别检测样品中单一药物的残留量，难以客观地反映出孔雀石绿 和隐孔雀石绿的总残留量。目前同时检测两种结构的免疫分析方法鲜有报道，本文分别制备了针对孔雀石绿和隐孔雀石绿的高特异性 单克隆抗体，通过研究混合抗体模式建立了可以同时检测两种物质的酶联免疫分析方法，该方法对孔雀石绿和隐孔雀石绿混合物的半 抑制浓度（50% inhibiting concentration, IC50 ）为3.27 ng/mL，检测