成纤维细胞生长因子7及其受体2Ⅲb在小鼠肾发育中的表达及汇编.docVIP

成纤维细胞生长因子7及其受体2Ⅲb在小鼠肾发育中的表达 田娟 李佳 郭敏△ （辽宁医学院组织学与胚胎学教研室，辽宁 锦州 121001） 摘要：目的 观察成纤维细胞生长因子7 (fibroblast growth factor 7，FGFⅢb (fibroblast growth factor receptor 2 Ⅲb ,FGFR2 Ⅲb）在小鼠肾发育中的时空性表达，探讨其与肾发育的关系。方法 选取胚龄E12、14、16、18d胎鼠和生后N1、3、7、14、21和42d仔鼠肾脏，应用免疫组织化学技术对小鼠肾组织中FGF7及其受体2 Ⅲb表达进行定位观察；应用体视学方法及免疫印迹技术对小鼠肾组织中FGF7及其受体2 Ⅲb表达进行定性分析和定量检测。结果 免疫组织化学结果显示，E12d FGF7和FGFR2Ⅲb开始在输尿管芽微弱表达；以后随着肾脏发育成熟，FGF7和FGFR2Ⅲb主要表达于远端小管和集合管，在近端小管、生后肾组织及发育各阶段肾小体未见阳性表达。体视学和免疫印迹结果显示，从E14d至N7d，FGF7和FGFR2Ⅲb在肾脏的表达量明显增加，N7d至N42d表达量逐渐减少。结论 FGF7和FGFR2Ⅲb对肾脏远端小管和集合管的发生发育可能起重要作用。 关键词： FGF7；FGFR2Ⅲb；肾发育；小鼠 中国图书资料分类号：R329.461 文献标识码：A 文章编号： Expression of FGF 7 and FGFR 2Ⅲb in development of mouse kidney TIAN Juan, LI Jia, GUO Min△ (Department of Histology and Embryology, Liaoning Medical College, Jinzhou 121001 China) Abstract: Objective To observe the temporal and spatial expressions of fibroblast growth factor 7 （FGF7）and fibroblast growth factor receptor 2 Ⅲb （FGFR2 Ⅲb）during kidney development of mouse, and investigate the relationship between FGF7、FGFR2 Ⅲb and kidney development. Methods The expressions of FGF7 and FGFR2Ⅲb were examined by immunohistochemistry. The protein content of FGF7 and FGFR2Ⅲb in embryonic(including fetal age of 12,14,16 and 18d) and neonatal(including the age of 1,3,7,14,21 and 42d)mouse kidneys were measured by 作者简介：田娟，讲师，博士在读研究生。主要从事肾脏发育生物学方面的研究。E-mail：tian555juan555@ 通讯作者：郭敏 E-mail: mguo1118@ 基金项目：辽宁省教育厅科学技术研究项目（L2010274）；辽宁医学院青年科技启动基金项目（Y2010Z005） Western blot and stereological method. Results Immunohistochemical analysis showed that FGF7 and FGFR2 IIIb were weakly expressed in the ureteric buds at E12d and then with the renal development, FGF7 and FGFR2 IIIb were expressed mainly in distal tubules and collecting ducts. FGF7 and FGFR2Ⅲb was not expressed in the proximal tubule. The renal corpuscles in each development stage were not found the expression of FGF7 and FGFR2 IIIb. The results of Western blot and stereological method showed that from E14 to N7, the expre