康照菌剂H对魔芋的抗病促生作用-应用与环境生物学报.DOC

链霉菌剂对魔芋的促生防病作用* 何 斐1 张忠良2 崔 鸣3 薛泉宏4**1西北农林科技大学生命科学学院陕西杨陵 7121002西北农林科技大学林学院陕西杨陵 7121003秦巴魔芋研究开发中心陕西安康 7250004西北农林科技大学资源环境学院陕西杨陵 712100摘 要通过皿内试验研究生防链霉菌Act1肉质链霉菌Streptomyces carnosus）和Act12（密旋链霉菌Streptomyces pctum）对软腐病菌的拮抗及链霉菌无细胞发酵液对甜瓜种子萌发及幼苗生长的影响；采用盆栽试验研究生防菌剂Act11和Act12按质量比1:1的固态发酵混合制剂）对魔芋软腐病的防效以及对植株光合、生物量、产量及球茎品质的影响。结果表明：①生防链霉菌Act12无细胞发酵滤液对6种软腐病菌均有拮抗作用，拮抗圈直径19.4～31.6 mm；无细胞能促进甜瓜种子萌发和幼苗生长。②链霉菌活菌剂对魔芋软腐病有一定防效。菌剂拌土单独接种对收获时魔芋软腐病的相对防效为65.0%；菌剂与软腐病菌混接处理，在病原菌接种后第10、20 d的相对防效分别为100.0%58.4%。③链霉菌剂能促进魔芋生长，提高产量。菌剂与软腐病菌混接处理魔芋地径、净光合速率、球茎鲜、根茎鲜及根茎分别较软腐病菌单接显著增加11.1%、111.7%、54.8%、304.2%及200.0%（P0.05）。④链霉菌剂接种能明显改善魔芋球茎品质。菌剂单接处理魔芋球茎葡甘聚糖、淀粉及纤维素含量分别较对照显著增加14.0%、8.1%及27.7%（P0.05）。结论：接种链霉菌剂明显促进魔芋生长，提高魔芋产量和葡甘聚糖含量，增强抗病能力。 关键词魔芋；软腐病；链霉菌剂；促生；防病 A S144.2; S154.39 Growth promotion and disease prevention of streptomyces on Amorphophallus konjac K. Koch ex N.E.Br* HE Fei1, ZHANG Zhongliang2, CUI Ming3 XUE Quanhong4** 1College of Life Science, Northwest AF University, Yangling Shaanxi 712100, China 2College of Forestry, Northwest AF University, Yangling Shaanxi 712100, China 3Qinba Konjac Research and Development Center, Ankang Shaanxi 725000, China 4College of Natural Resources and Environment, Northwest AF University, Yangling Shaanxi 712100, China Abstract The antagonism and seed growth promotion effect were evaluated by agar block and axenic fermentation extract experiments. Pot experiments were taken to assess relative biocontrol effiency and effect of streptomyce preparations M (mixture of S. carnosus and S. pctum in type of solid-state fermentation with 1:1 ratio) on biomass, photosynthetic rate, yield and corm quality of A. konjac K. Koch ex N.E.Br. Results showed that: ① Streptomyces Act12 showed obvious antagonistic activity against six soft rot pathogens, and the diameter of growth inhibition zones ranged from 19.4 to 31.6 mm. And fermentation filtrate of streptomyces exhibited growth-promoting activity on melon seed germination and seedling growth; ② Streptomyce-derived viable