微囊藻毒素的提取和提纯研究-环境科学学报.PDF

第 24 卷第 2 期 环 　境 　科 　学 　学 　报 Vol . 24 ,No . 2 2004 年 3 月 ACTA SCIENTIAE CIRCUMSTANTIAE Mar . ,2004 ( ) 文章编号 2004 　　　中图分类号 :Q9333 　　　文献标识码 :A 微囊藻毒素的提取和提纯研究 闫　海 ,潘 　纲 ,张明明 ,陈海柳 ,邹 　华　( 中国科学院生态环境研究中心 ,环境水质学国 家重点实验室 ,北京 　100085) ( ) 摘要 :对采 自云南滇池水华蓝藻细胞中的微囊藻毒素 Microcystins ,MCs 的提取与提纯方法进行了研究. 结果表明,在藻细 胞干重浓度为 20 g ·L - 1 下 ,与不同甲醇浓度提取液相比,40 % 甲醇溶液可以最有效地从藻细胞中提取出MCRR 和 MCLR. 将 MCs 提取液过 Waters 固相萃取小柱后 ,用 70 % 甲醇溶液洗脱吸附于柱上的MCs ,可以分别获得 73 %和 35 %纯度的MCRR 和MCLR. 通过观察洗脱液的颜色变化 , 收集蓝绿色和橘黄色后面流出的基本无色的洗脱液 , 可以获得纯度为 286 %和 129 %的MCRR 和 MCLR. 关键词 :微囊藻毒素 ;提取 ;提纯 Study on the extraction and purif ication of microcystins ( YAN Hai , PAN Gang , ZHANG Mingming ,CHEN Hailiu , ZOU Hua 　 State Key Laboratory of Environmental Aquatic Chemistry , Research Center for Ecoenvironmental Sciences , Chinese Academy of Sciences , Beijing 　100085) Abstract :The methods for extracting and purifying microcystin (MCs) in dry cyanobacterial cells taken from Dianchi Lake , Yunnan Province , were studied in this paper . 40 % methanolwater solution was found to be an optimum solution for effectively extracting MCRR and MCLR - 1 from cyanobacterial cells among different ratios of methanol when the concentration of dry cyanobacterial cells was 20 g ·L . After the extrac tion solution of 40 % methanol containing MCs was passed through a cartridge (Waters , OASISTM HLB Cartridge) , MCs were purified by the elution with 70 % methanol , and 73 % and 35 % purities of MCRR and MCLR were obtained. By c