生鲜猪肉低场核磁检测参数优化-食品科学.pdfVIP

※安全检测 食品科学 2015, Vol.36, No.12 177 生鲜猪肉低场核磁检测参数优化 * 王志永，王 欣 ，夏义苗，黄远芬，刘宝林 （上海理工大学食品质量与安全研究所，上海 200093 ） 摘  要：低场核磁共振（low field nuclear magnetic resonance ，LF-NMR ）技术在反映肉品内部信息上具有显著优 势，而合适的检测参数对于测定结果的准确性提高检测效率至关重要。以生鲜猪精瘦肉为对象，对其LF-NMR检 测中的检测参数，如重复采样等待时间、半回波时间、回波个数、重复扫描次数、样品质量及温度等进行优化， 并进行了验证。结果表明，取质量4～5 g 的样品于32 ℃水浴至恒温后，在重复采样等待时间4 500 ms 、半回波时间 200 μs、回波个数3 000、重复扫描次数8、采样频率250 kHz 的条件下采样，有利于获得良好稳定性与重复性的检测 结果。所得条件可用于猪肉的LF-NMR相关研究，亦可为其他肉制品在LF-NMR 中的研究提供借鉴。 关键词：猪肉；低场核磁共振；参数优化 Assessment of Fresh Pork Quality by LF-NMR: Parameter Optimization * WANG Zhiyong, WANG Xin , XIA Yimiao, HUANG Yuanfen, LIU Baolin (Institute of Food Safety and Quality, University of Shanghai for Science and Technology, Shanghai 200093, China) Abstract: Information about water mobility and distribution in meat can be provided by proton nuclear magnetic resonance (NMR) relaxometry. The suitable measurement parameters of low-field proton nuclear magnetic resonance (LF-NMR) relaxometry will not only be helpful for the reliability of experiment data, which reflect accurately the information on the sample, but also be beneficial for enhancing the detection efficiency. In this study, using fresh lean pork from the hind legs, the effects of LF-NMR parameters on the accuracy, stability and reproducibility of the experimental results were investigated. No significant changes in experimental data (P 0.05) were observed when the tR (repetition delay) and Echo Count (sampling data number) were set as more than 4 500 ms and more than 3 000, respectively. The optimal conditions were determine as follows: τ value (time between 90 d