微管相关蛋白-1B在弥漫性轴索损伤中的表达变化与意义.docVIP

微管相关蛋白-1B在弥漫性轴索损伤中的表达变化与意义 张 鹏1，赵敏珠1,2，朱士胜3，刘 梦1,2，高 军1,2，李红卫2,4，李剑波1 重庆医科大学基础医学院法医学教研室，重庆 400016；2.重庆市刑事侦查技术研究中心，重庆 400016；3.重庆医药高等专科学校，重庆 401331；4.重庆市公安局刑警总队技术处，重庆 400021） 【摘 要】目的：观察微管相关蛋白-1B（microtubule-associate protein 1B，MAP1B）在SD大鼠弥漫性轴索损伤（diffuse axonal injury ，DAI）后脑组织中表达的变化，探索其在DAI中的作用机制。方法：采用改良的Marmarou法建立SD大鼠DAI模型，分为空白对照组（不予任何处理），假打击组（给予致伤组相同的打击前准备，但不予以打击），致伤1 d组和致伤3 d组，于打击1 d后、3 d后，处死动物，提取脑组织，结合western blot和免疫组化方法，观察SD大鼠脑组织中MAP1B表达的变化。结果：致伤1 d组和致伤3 d组中MAP1B表达水平较空白对照组、假打击组明显升高。结论：SD大鼠DAI后脑组织中MAP1B表达水平上调，提示其参与了DAI后轴索损伤的病理改变过程，并可能在稳定、修复及再生受损轴索的机制中起着重要作用。 【关键词】弥漫性轴索损伤；微管相关蛋白-1B；轴索修复；SD大鼠 Expression and significance of Microtubule-associate protein 1B in rats with diffuse axonal injury Zhang Peng1，Zhao Minzhu1,2，Zhu Shisheng3，Liu Meng1,2，Gao Jun1,2，Li Hongwei2,4, Li Jianbo1 (1. Department of Forensic medicine, Chongqing Medical University; 2. Chongqing Engineering Research Center for Criminal Investigation Technology, Chongqing; 3. Faculty of Medical Technology, Chongqing Medical and Pharmaceutical College; 4.Technical Department of Interpol Corps of the Chongqing Police Bureau,Chongqing) 【Abstract】Objective: To examine the expression of the microtubule-associate protein 1B (MAP1B) in rats with diffuse axonal injury (DAI) as well as to elucidate the mechanisms of MAP1B in the procession of axonal repairment. Methods: DAI was induced in adult male SD rats using an injury model adapted from Marmarou. The rats were divided into four groups: injured 1 d group (n=10), injured 3 d group (n=10), sham group (n=10) and sham-injured group (n=10). The animals were sacrificed at 1 d and 3 d post injury. Western blot analyses and immunohistochemistry analyses were performed to observe the expression of MAP1B in rats with DAI. Results: Comparing with sham group and sham-injured group, MAP1B was upexpressed in the brain tissues of injury group. Conclusions: Expression of MAP1B was increased in the brain tissues of injury group, which indicates MAP1B may be involved in the