响应曲面法优化山蕗菜根多糖的提取工艺.kdh.pdfVIP

46 2010, Vol. 31, No. 02 食品科学 ※工艺技术 响应曲面法优化山蕗菜根多糖的提取工艺 王 鸿，邓泽元* ，刘 蓉，范亚苇，李 静 (南昌大学食品科学与技术国家重点实验室，高等研究院，江西南昌 33004 7) 摘 要：以山蕗菜根提取类黄酮后的残渣为材料，研究山蕗菜根多糖的提取工艺。在单因素试验基础上，选定提 取温度、时间和料液比3 个因素的3 个水平进行中心组合试验，建立多糖提取率的二次回归方程，通过响应面分 析得到优化组合条件。结果表明：提取工艺条件为提取温度70 ℃、提取时间2 .3h 、料液比1:4 5(g/mL) 、提取1 次 时，多糖提取率达到最大值。该条件下多糖提取率预测值为3 5 .04 % ，验证值为34 .74 % 。 关键词：山蕗菜根；多糖；提取工艺；响应曲面法优化 Optimization of Extraction Technology of Polysaccharides from Petasites tricholobus Roots by Response Surface Analysis WANG Hon g ，DENG Ze-yu an * ，LIU Ron g ，FAN Ya-wei ，LI Jing (State Key Laboratory of Food Science and Technology, In stitute of Advanced Studies, Nanchang University, Nanchang 330047, China) Abstract ：Crude poly saccharides were prepared from the residue of Petasites tricholobus roots after flavonoids extraction. According to single-factor experiments, three levels of the factors including extraction temperature, extraction time and liquid/solid ratio were selected for the central composite design to establish a quadric regression equation for predicting the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction tempera- ture 70 ℃, extraction time 2.3 h and liquid/solid ratio 45:1, which led to estimated and observed values of maximal yield of polysaccharides of 35.04% and 34 .74%, respectively. Key words ：P etasites tricholobus roots ；poly saccharides ；extraction technology ；respon se surface methodology 中图分类号：TS255.36 文献标识码：A 文章编号：1002-6630(20 10)02-0046-05 山蕗菜，中文学名掌叶蜂斗菜( P e t a s i t e s 酮与多糖等综合开发的道路，提高山蕗菜根资源的综合 trich olobus A . Gr ay