altered regulation of contraction-induced aktmtorp70s6k pathway signaling in skeletal muscle of the obese zucker rat改变监管contraction-induced aktmtorp70s6k通路信号zucker肥胖大鼠的骨骼肌.pdfVIP

Hindawi Publishing Corporation Experimental Diabetes Research Volume 2009, Article ID 384683, 9 pages doi:10.1155/2009/384683 Research Article Altered Regulation of Contraction-Induced Akt/mTOR/p70S6k Pathway Signaling in Skeletal Muscle of the Obese Zucker Rat Anjaiah Katta,1, 2 Sunil Kakarla,1, 2 Miaozong Wu,2, 3 Satyanarayana Paturi,2, 3 Murali K. Gadde,2, 3 Ravikumar Arvapalli,2, 3 Madhukar Kolli,1, 2 Kevin M. Rice,2, 3, 4 and Eric R. Blough1, 2, 3, 4, 5 1 Department of Pharmacology, Physiology, and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA 2 Cell Diff erentiation and Development Center, Marshall University, Huntington, WV 25755, USA 3 Department of Biological Sciences, Marshall University, Huntington, WV 25755, USA 4 Laboratory of Molecular Physiology, Marshall University, 241N Byrd Biotechnology Building, 1 John Marshall Drive, Huntington, WV 25755-1090, USA 5 Department of Exercise Science, Sport and Recreation, College of Education and Human Services, Marshall University, Huntington WV 25755, USA Correspondence should be addressed to Eric R. Blough, blough@marshall.edu Received 13 March 2009; Revised 15 July 2009; Accepted 15 December 2009 ¨ Recommended by Andreas Pfutzner Increased muscle loading results in the phosphorylation of the 70 kDa ribosomal S6 kinase (p70S6k), and this event is strongly correlated with the degree of muscle adaptation following resistance exercise. Whether insulin resistance or the comorbidities associated with this disorder may affect the ability of skeletal muscle to activate p70S6k signaling following an exercise stimulus remains unclear. Here, we compare the contraction-induced activation of p70S6k signalin