antioxidant activities and phytochemical study of leaf extracts from 18 indigenous tree species in taiwan抗氧化活动和植物化学的研究叶提取物18日在台湾本土树种.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 215959, 8 pages doi:10.1155/2012/215959 Research Article Antioxidant Activities and Phytochemical Study of Leaf Extracts from 18 Indigenous Tree Species in Taiwan Shang-Tse Ho,1 Yu-Tang Tung,2 Yong-Long Chen,1 Ying-Ying Zhao,1 Min-Jay Chung,3 and Jyh-Horng Wu1 1 Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan 2 Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan 3 The Experimental Forest, National Taiwan University, Taipei 106, Taiwan Correspondence should be addressed to Jyh-Horng Wu, eric@.tw Received 30 June 2011; Accepted 25 October 2011 Academic Editor: Virginia S. Martino Copyright © 2012 Shang-Tse Ho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The objective of this study is to assess antioxidant activities of methanolic extracts from the leaves of 18 indigenous tree species in Taiwan. Results revealed that, among 18 species, Acer oliverianum exhibited the best free radical scavenging activities. The IC50 values were 5.8 and 11.8 μg/mL on DPPH radical and superoxide radical scavenging activities, respectively. In addition, A. olive- rianum also exhibited the strongest ferrous ion chelating activity. Based on a bioactivity-guided isolation principle, the resulting methanolic crude extracts of A. oliverianum leaves were fractionated to yield soluble fractions of hexane, EtOAc, BuOH, and water. Of these, the EtOAc fraction had the best antioxidant activity. Furthermore, 8 specific phytochemicals were isolated and identified