anti-obesity and anti-diabetic effects of acacia polyphenol in obese diabetic kkay mice fed high-fat diet肥胖和金合欢属植物多酚的抗糖尿病影响肥胖糖尿病kkay老鼠喂食高脂肪的饮食.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 952031, 10 pages doi:10.1093/ecam/nep241 Original Article Anti-Obesity and Anti-Diabetic Effects of Acacia Polyphenol in Obese Diabetic KKAy Mice Fed High-Fat Diet Nobutomo Ikarashi, Takahiro Toda, Takehiro Okaniwa, Kiyomi Ito, Wataru Ochiai, and Kiyoshi Sugiyama Department of Clinical Pharmacokinetics, Hoshi University, Tokyo, Japan Correspondence should be addressed to Kiyoshi Sugiyama, sugiyama@hoshi.ac.jp Received 13 October 2009; Accepted 12 December 2009 Copyright © 2011 Nobutomo Ikarashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Acacia polyphenol (AP) extracted from the bark of the black wattle tree (Acacia meansii ) is rich in unique catechin-like flavan- 3-ols, such as robinetinidol and fisetinidol. The present study investigated the anti-obesity/anti-diabetic effects of AP using obese diabetic KKAy mice. KKAy mice received either normal diet, high-fat diet or high-fat diet with additional AP for 7 weeks. After the end of administration, body weight, plasma glucose and insulin were measured. Furthermore, mRNA and protein expression of obesity/diabetic suppression-related genes were measured in skeletal muscle, liver and white adipose tissue. As a result, compared to the high-fat diet group, increases in body weight, plasma glucose and insulin were significantly suppressed for AP groups. Furthermore, compared to the high-fat diet group, mRNA expression of energy expenditure-related genes (PPARα, PPARδ, CPT1, ACO and UCP3) was significantly higher for AP groups in skeletal muscle. Prot