antioxidant activity of lawsonia inermis extracts inhibits chromium(vi)-induced cellular and dna toxicity抗氧化活性的lawsonia inermis提取物抑制铬(vi)全身的细胞和基因毒性.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 576456, 9 pages doi:10.1093/ecam/nep205 Original Article Antioxidant Activity of Lawsonia inermis Extracts Inhibits Chromium(VI)-Induced Cellular and DNA Toxicity Gunjan Guha, V. Rajkumar, R. Ashok Kumar, and Lazar Mathew School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore 632 014, India Correspondence should be addressed to R. Ashok Kumar, ashoku 2000@ Received 18 June 2009; Accepted 9 November 2009 Copyright © 2011 Gunjan Guha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Hexavalent chromium Cr(VI) is a very strong oxidant which consequently causes high cytotoxicity through oxidative stress. Prevention of Cr(VI)-induced cellular damage has been sought in this study in aqueous and methanolic extracts of Lawsonia inermis Linn. (Lythraceae), commonly known as Henna. The extracts showed significant (P .05) potential in scavenging free radicals (DPPH• and ABTS•+ ) and Fe3+ , and in inhibiting lipid peroxidation. DNA damage caused by exposure of pBR322 to Cr(VI)-UV is markedly inhibited by both extracts in varying degrees. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S (human breast carcinoma) cells with an increase in dosage of both extracts individually. Furthermore, both extracts proved to contain a high content of phenolic compounds which were found to have a strong and significant (P .05) positive correlation to the radical scavenging potential, lipid peroxidation inhibition capacity and cyto-protective efficiency against