application of 2-trichloromethylbenzimidazole in analytical chemistry a highly selective chromogenic reagent for thin-layer chromatography and some other analytical uses2-trichloromethylbenzimidazole在分析化学中的应用具有高度选择性的显色试剂对薄层色谱法和其他一些分析使用.pdfVIP

Hindawi Publishing Corporation Journal of Analytical Methods in Chemistry Volume 2012, Article ID 650150, 10 pages doi:10.1155/2012/650150 Review Article Application of 2-Trichloromethylbenzimidazole in Analytical Chemistry: A Highly Selective Chromogenic Reagent for Thin-Layer Chromatography and Some Other Analytical Uses Leszek Konopski and Anna Kiełczewska Analytical Department, Institute of Industrial Organic Chemistry, Annopol 6, 03-236 Warsaw, Poland Correspondence should be addressed to Leszek Konopski, konopski@ipo.waw.pl Received 30 November 2011; Accepted 11 January 2012 Academic Editor: Josep Esteve-Romero Copyright © 2012 L. Konopski and A. Kiełczewska. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2-Trichloromethylbenzimidazole (TCMB) was used as a chromogenic reagent in organic or inorganic analysis, mainly in thin-layer chromatography (TLC). In reactions of TCMB with some heteroaromatic nitrogen containing compounds, such as azines, azoles and benzazoles, a formation of high colored products occurred. For azines, the chromogenic reaction was highly regioselective, since the both adjacent α-positions versus the nitrogen atom(s) must not be substituted. A TLC method of detection was developed. Thirty azines, azoles, and benzazoles were detected at the detection limit 10 ng to 1 μg. This method was also applied for detection of heteroaromatic pesticides, and the attempts to construct active and passive dosimeters for nicotine were made. In a prechromatographic reaction of aromatic o-diamines with methyl trichloroacetimidate, TCMB or its deriv