application of attenuated total reflection infrared spectroscopy in the study of peruphasma schultei defensive secretion衰减全反射红外光谱法的应用研究peruphasma schultei防守分泌.pdfVIP

Spectroscopy 21 (2007) 169–176 169 IOS Press Application of attenuated total reflection infrared spectroscopy in the study of Peruphasma schultei defensive secretion Michael P. McLeod a , Aaron T. Dossey b and M. Khalique Ahmed a,∗ a Lynn University, College of Arts and Sciences, 3601 North Military Trail, Boca Raton, FL 33463, USA b Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610-0245, USA Abstract. In this short communication, we present the first Fourier Transform Infrared Absorbance (FT-IR) study of peruphas- mal; a defensive secretion from Peruphasma schultei. The spectral data collected are representative of the natural product structure proposed by Dossey et al. This study demonstrates the viability of FT-IR as another tool in the physical and biological chemist’s repertoire for use in determining important structural characteristics from minute amounts of available sample. Keywords: Peruphasmal, secretion, nuclear magnetic resonance, infra-red spectrum assignments, FT-IR, NMR 1. Introduction Individual organisms often produce natural product compounds in extremely minute amounts. How- ever, relatively large quantities of these products are typically needed for meaningful structural studies [1–3]. Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for structural studies [3,4], but has a large sample requirement [2,3]. Recently, Dossey et al. [2] used a special 1.0-mm diameter NMR probe, invented by Brey et al. [4], that significantly reduces the sampling requirement for NMR [2]. Dossey et al. [2] were able to use this technology to study the defensive secretions from individ- ual walkingstick insects (Order Phasmatodea). Another physical technique that can be used to study the structures of natural products is Fourier Transform Infrared Spectroscopy (FT-IR) [5,6]. Recent