aquaporin-1-mediated effects of low level he-ne laser irradiation on human erythrocytesaquaporin-1-mediated低强度氦氖激光照射对人体红细胞的影响.pdfVIP

Hindawi Publishing Corporation International Journal of Photoenergy Volume 2012, Article ID 275209, 5 pages doi:10.1155/2012/275209 Research Article Aquaporin-1-Mediated Effects of Low Level He-Ne Laser Irradiation on Human Erythrocytes Gang-Yue Luo,1, 2, 3, 4 Li Sun,5 and Timon Cheng-Yi Liu1, 2, 3 1 Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510631, China 2 School of Life Science, South China Normal University, Guangzhou 510631, China 3 The Key Laboratory of Ministry of Education of China in Laser Life Science, South China Normal University, Guangzhou 510631, China 4 School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China 5 Medical School, Jinan University, Guangzhou 510632, China Correspondence should be addressed to Timon Cheng-Yi Liu, liutcy@ Received 30 January 2012; Revised 12 April 2012; Accepted 12 April 2012 Academic Editor: Rui Duan Copyright © 2012 Gang-Yue Luo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The role of membrane aquaporin-1 (APQ-1) in the photobiomodulation (PBM) on erythrocyte deformability will be studied in this paper with human dehydrated erythrocytes as echinocytic shape alterations lead to decreased cellular deformability. Human dehydrated erythrocytes were irradiated with low intensity He-Ne laser irradiation (LHNL) at 0.9, 1.8, 2.7, and 4.4 mW/cm2 for 5, 15, and 30 min, respectively, and APQ-1 inhibitor, 0.2 µmol/L HgCl2 , was used to study the role of APQ-1 in mediating PBM with LHNL at 4.4 mW/cm2 for 5 min. Comprehensive morphological pa