bovine serum albumin antibodies as a disease marker for hepatitis e virus infection牛血清白蛋白抗体作为e型肝炎病毒感染的疾病标记.pdfVIP

Journal of Biomedicine and Biotechnology • 2005:4 (2005) 316–321 • DOI: 10.1155/JBB.2005.316 RESEARCH ARTICLE Bovine Serum Albumin Antibodies as a Disease Marker for Hepatitis E Virus Infection Medhat Haroun Department of Bioscience Technology, Institute of Graduate Studies Research, Alexandria University, 163 Horreya Avenue, PO Box 832, Chatby 21526, Alexandria, Egypt Received 4 March 2005; revised 1 June 2005; accepted 7 June 2005 This report evaluates the significance of antibody/bovine serum albumin (BSA) interactions as a risk factor for the diagnosis of acute hepatitis E. Serum samples from 40 patients with acute hepatitis E and from 40 age/sex matched healthy adult subjects were tested for IgA, IgG, and IgM by ELISA and by turbidimetric assay. BSA was used as a target to characterize changes in levels of interacting immunoglobulins. Initial results obtained before removal of antibodies that interacted with BSA suggested that HEV patients had increased levels of IgM in their sera. It was found that normal individuals had mean IgA, IgG, and IgM levels of 2.55 mg/mL, 9.80 mg/mL, and 1.73 mg/mL, respectively while HEV patients had mean levels of 2.66 mg/mL, 10.04 mg/mL, and 2.01 mg/mL (P .26, P .32, and P .0004). However, the mean level of IgM in HEV-infected sera after purification from antibodies that interacted with BSA was determined to be 1.72 mg/mL indicating that there was no significant difference in IgM level in HEV patients compared to normal individuals (P .6). The presence of antibodies that interact with BSA might serve as a diagnostic tool for detection of high-risk patients. INTRODUCTION for the diagnosis of HEV infe