bioassay-guided isolation of cytotoxic cycloartane triterpenoid glycosides from the traditionally used medicinal plant leea indica获得孤立的细胞毒性cycloartane三萜苷从传统药用植物leea籼稻.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 164689, 11 pages doi:10.1155/2012/164689 Research Article Bioassay-Guided Isolation of Cytotoxic Cycloartane Triterpenoid Glycosides from the Traditionally Used Medicinal Plant Leea indica Yau Hsiung Wong,1 Habsah Abdul Kadir,1 and Sui Kiong Ling2 1 Biomolecular Research Group, Biochemistry Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Division of natural products, Forest Research Institute of Malaysia (FRIM), 52109 Kepong, Malaysia Correspondence should be addressed to Habsah Abdul Kadir, aakhak@ Received 16 May 2011; Revised 18 August 2011; Accepted 30 August 2011 Academic Editor: Cheorl-Ho Kim Copyright © 2012 Yau Hsiung Wong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Leea indica is a medicinal plant used traditionally to cure cancer. In this study, the cytotoxic compounds of L. indica were isolated using bioassay-guided approach. Two cycloartane triterpenoid glycosides, mollic acid arabinoside (MAA) and mollic acid xyloside (MAX), were firstly isolated from L. indica. They inhibited the growth of Ca Ski cervical cancer cells with IC50 of 19.21 μM (MAA) and 33.33 μM (MAX). MRC5 normal cell line was used to calculate selectivity index. MAA and MAX were about 8 and 4 times more cytotoxic to Ca Ski cells compared to MRC5. The cytotoxicity of MAA was characterized by both cytostatic and cytocidal effects. MAA decreased the expression of proliferative cell nuclear antigen, increased sub-G1 cells, and arrested cells in