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Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 973140, 8 pages doi:10.1155/2012/973140 Research Article BAC-Dkk3-EGFP Transgenic Mouse: An In Vivo Analytical Tool for Dkk3 Expression Yuki Muranishi1, 2 and Takahisa Furukawa1, 2, 3 1JST, CREST, 3-2 Yamadaoka, Osaka, Suita 565-0871, Japan 2 Department of Developmental Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Osaka, Suita 565-0874, Japan 3 Laboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita 565-0871, Japan Correspondence should be addressed to Takahisa Furukawa, takahisa.furukawa@protein.osaka-u.ac.jp Received 15 February 2012; Revised 19 April 2012; Accepted 3 May 2012 Academic Editor: Thomas Lufkin Copyright © 2012 Y. Muranishi and T. Furukawa. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Dickkopf (DKK) family proteins are secreted modulators of the Wnt signaling pathway and are capable of regulating the development of many organs and tissues. We previously identified Dkk3 to be a molecule predominantly expressed in the mouse embryonic retina. However, which cell expresses Dkk3 in the developing and mature mouse retina remains to be elucidated. To examine the precise expression of the Dkk3 protein, we generated BAC-Dkk3-EGFP transgenic mice that express EGFP integrated into the Dkk3 gene in a BAC plasmid. Expression analysis using the BAC-Dkk3-EGFP transgenic mice revealed that Dkk3 is ¨