brain levels of catalase remain constant through strain, developmental, and chronic alcohol challenges大脑的过氧化氢酶水平通过应变保持不变,发展,和慢性酒精的挑战.pdfVIP

Hindawi Publishing Corporation Enzyme Research Volume 2012, Article ID 572939, 6 pages doi:10.1155/2012/572939 Research Article Brain Levels of Catalase Remain Constant through Strain, Developmental, and Chronic Alcohol Challenges Dennis E. Rhoads, Cherly Contreras, and Salma Fathalla Biology Department, Monmouth University, West Long Branch, NJ 07764, USA Correspondence should be addressed to Dennis E. Rhoads, drhoads@ Received 30 April 2012; Accepted 28 June 2012 Academic Editor: H. Kuhn Copyright © 2012 Dennis E. Rhoads et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Catalase (EC ) oxidizes ethanol to acetaldehyde within the brain and variations in catalase activity may underlie some consequences of ethanol consumption. The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings. In the first series of studies, levels of catalase were compared between juvenile and adult rats and between the Long-Evans (LE) and Sprague-Dawley (SD) strains. Levels of catalase appear to have achieved the adult level by the preadolescent period defined by postnatal age (P, days) P25–P28, and there were no differences between strains at the developmental stages tested. Thus, variation in catalase activity is unlikely to be responsible for differences in how adolescent and adult rats respond to ethanol. In the second series of studies, periadolescent and adult rats were administered ethanol chronically through an ethanol-containing liquid diet. Diet consumption and blood ethanol concentrations were signifi