tyrosinase-immobilized biosensor based on the functionalized hydroxyl group-mwnt and detection of phenolic compounds in red wines功能化羟基group-mwnt tyrosinase-immobilized生物传感器的基础上,检测红酒的酚类化合物.pdf

Hindawi Publishing Corporation Journal of Sensors Volume 2009, Article ID 916515, 9 pages doi:10.1155/2009/916515 Research Article Tyrosinase-Immobilized Biosensor Based on the Functionalized Hydroxyl Group-MWNT and Detection of Phenolic Compounds in Red Wines Ji-Hoon Yang, Jae-Chan Lee, and Seong-Ho Choi Department of Chemistry, BK 21 NanoBiosensor Research Team, Hannam University, Daejeon 305-811, South Korea Correspondence should be addressed to Seong-Ho Choi, shchoi@hnu.kr Received 24 July 2009; Accepted 31 August 2009 Recommended by Kourosh Kalantar-Zadeh The tyrosinase-immobilized biosensor was developed with the hydroxyl group-functionalized multiwall carbon nanotube (MWNT) for phenol detection. The hydroxyl group-modified MWNT was modified to include poly(GVPB)-g-MWNT, or poly(HEMA), by a radiation-induced graft polymerization of glucosyl 4-vinylphenylboronate (GVPB) or 2-hydroxyethyl- methacrylate (HEMA) on the surface of MWNT. The response of biosensor was in the range of 0.6–7.0 mM for concentration and in the range of 0.05–0.35 mM for phenol in a phosphate buffer solution, respectively. Various parameters influencing biosensor performance have been optimized: for pH, temperature, and the response to various phenolic compounds. The biosensor was then tested on phenolic compounds contained in three different commercial red wines. Copyright © 2009 Ji-Hoon Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 1. Introduction acrylic acid and methacrylic acid to increase the affinity of