screening and phylogenetic analysis of deep-sea bacteria capable of metabolizing lignin-derived aromatic compounds筛查和系统发育分析深海细菌代谢lignin-derived芳香族化合物的能力.pdf

Open Journal of Marine Science, 2012, 2, 177-187 /10.4236/ojms.2012.24021 Published Online October 2012 (http://www.SciRP.org/journal/ojms) Screening and Phylogenetic Analysis of Deep-Sea Bacteria Capable of Metabolizing Lignin-Derived Aromatic Compounds * Y. Ohta , S. Nishi, T. Haga, T. Tsubouchi, R. Hasegawa, M. Konishi, Y. Nagano, Y. Tsuruwaka, Y. Shimane, K. Mori, K. Usui, E. Suda, K. Tsutsui, A. Nishimoto, Y. Fujiwara, T. Maruyama, Y. Hatada Institute of Biogeosciences, Japan Agency of Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan * Email: ohtay@jamstec.go.jp Received August 15, 2012; revised September 15, 2012; accepted September 30, 2012 ABSTRACT Lignin is one of the most abundant biomasses in nature. It is composed of aromatic moieties and has great potential for use in the production of chemical alternatives to petroleum products. Because of increasing interest in biocatalysis, the potential for industrial application of microbial metabolism of lignin-derived compounds has gained considerable recent attention. Functional screenings of culturable bacteria isolated from sediments and sunken wood collected from the deep sea revealed the existence of a number of previously unidentified bacteria capable of metabolizing lignin-related aromatic compounds. Of the 510 isolates obtained in the present study, 208 completely or partially metabolized these compounds. The 208 isolates were classified into diverse phyla, including Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria. Among the 208 isolates, 61 unique 16S rRNA gene sequences were detected including