ssincc simple separation of interacting nucleoprotein complex componentsssincc简单分离核蛋白质复杂的组件进行交互.pdf

Advances in Biological Chemistry, 2012, 2, 146-151 ABC /10.4236/abc.2012.22017 Published Online May 2012 (http://www.SciRP.org/journal/abc/) SSINCC: Simple separation of interacting nucleoprotein complex components Roderick A. Slavcev, Nafiseh Nafissi, Tranum Kaur School of Pharmacy, University of Waterloo, Waterloo, Canada Email: slavcev@uwaterloo.ca Received 9 November 2011; revised 18 January 2012; accepted 21 March 2012 ABSTRACT nisms of unit copy plasmid segregation systems and it is just one of many areas of scientific investigation that Protein-DNA binding assays have been used in a va- employs such biochemical assays. Plasmids serve as riety of applications from fundamental studies re- tractable model systems to study DNA segregation be- garding the binding process itself to serve as probes cause they require only a DNA centromere, a centro- for the detection, quantification and separation of mere-binding protein and a force-generating ATPase. In target analytes. Here we describe a novel method of this study we describe a novel DNA-DNA capture analyzing and identifying intermolecular DNA inter- method, and use it to examine the ability of plasmid par- actions that allows for the simple separation of inter- tition proteins to pair plasmids at their centromeres. a