some observations on carbon nanotubes susceptibility to cell phagocytosis一些观察碳纳米管对细胞的吞噬作用.pdf
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some observations on carbon nanotubes susceptibility to cell phagocytosis一些观察碳纳米管对细胞的吞噬作用
Hindawi Publishing Corporation
Journal of Nanomaterials
Volume 2011, Article ID 473516, 8 pages
doi:10.1155/2011/473516
Research Article
Some Observations on Carbon Nanotubes Susceptibility to
Cell Phagocytosis
Aneta Fraczek-Szczypta,1 Elzbieta Menaszek,1, 2 and Stanislaw Blazewicz1
1 Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH-University of Science and Technology,
al Mickiewicza 30, 30-059 Krakow, Poland
2 Department of Cytobiology, Collegium Medicum, Jagiellonian University, Medyczna 9, 30-068 Krakow, Poland
Correspondence should be addressed to Aneta Fraczek-Szczypta, afraczek@.pl
Received 30 September 2010; Accepted 30 December 2010
Academic Editor: Xiaojun Yu
Copyright © 2011 Aneta Fraczek-Szczypta et al. This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
The aim of this study was to assess the influence of different types of carbon nanotubes (CNTs) on cell phagocytosis. Three
kinds of carbon nanotubes: single-walled carbon nanohorns (SWCNHs), multiwalled carbon nanotubes (MWCNTs), and ultra-
long single-walled carbon nanotubes (ULSWCNTs) before and after additional chemical functionalization were seeded with
macrophage cell culture. Prior to biological testing, the CNTs were subjected to dispersion process with the use of phosphate
buffered solution (PBS) and PBS containing surfactant (Tween 20) or dimethyl sulfoxide (DMSO). The results indicate that the
cells interaction with an individual nanotube is entirely different as compared to CNTs in the form of aggregate. The presence of the
surfactant favors the CNTs dispersion in culture media and facilita
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