stem cells grown in osteogenic medium on plga, plgaha, and titanium scaffolds for surgical applications干细胞生长在成骨的介质在plga,plgaha,钛支架手术的应用程序.pdf

Hindawi Publishing Corporation Bioinorganic Chemistry and Applications Volume 2010, Article ID 831031, 12 pages doi:10.1155/2010/831031 Research Article Stem Cells Grown in Osteogenic Medium on PLGA, PLGA/HA, and Titanium Scaffolds for Surgical Applications Annalia Asti,1, 2 Giulia Gastaldi,2, 3 Rossella Dorati,4 Enrica Saino,2, 5 Bice Conti,2, 4 Livia Visai,2, 5 and Francesco Benazzo1, 2 1 IRCCS Foundation, Orthopaedic and Traumatology Department, SMEC Department, San Matteo Hospital Institute, University of Pavia, 27100 Pavia, Italy 2 Center for Tissue Engineering (CIT), University of Pavia, Via Ferrata 1, 27100 Pavia, Italy 3 Department of Physiology, Section of Human Physiology, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy 4 Department of Pharmaceutical Chemistry, University of Pavia, Viale Taramelli, 27100 Pavia, Italy 5 Department of Biochemistry, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, Italy Correspondence should be addressed to Annalia Asti, a.asti@smatteo.pv.it Received 9 June 2010; Revised 15 September 2010; Accepted 27 October 2010 Academic Editor: Zheng Dong Copyright © 2010 Annalia Asti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts. We isolated hASCs from subcutaneous adipose tissue during orthopaedic surgery and induced the osteogenic differentiation for 28 days on three different synthetic scaffolds such as polylactide-co-glycolide (PLGA), polylactide-co- glycolide/hydroxyapatite (PLGA/HA),