stimulation of selected cellulases from trichoderma reesei with white linearly polarized light刺激的选择了多种纤维素酶木霉属reesei与白色线性偏振光.pdf

Open Journal of Organic Polymer Materials, 2012, 2, 45-52 /10.4236/ojopm.2012.23007 Published Online July 2012 (http://www.SciRP.org/journal/ojopm) Stimulation of Selected Cellulases from Trichoderma reesei with White Linearly Polarized Light 1 1 2 1 Ewelina Nowak , Gohar Khachatryan , Agnieszka Polit , Lidia Krzeminska-Fiedorowicz , Marta Dziedzicka-Wasylewska2, Maciej Fiedorowicz1* 1Department of Chemistry and Physics, University of Agriculture, Krakow, Poland 2Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland * Email: rrfiedor@.pl Received April 17, 2012; revised May 23, 2012; accepted June 4, 2012 ABSTRACT The impact of the illumination with white linearly polarized light (WLPL) of two commercially available cellulases from Trichoderma reesei on their activity in hydrolysis of microcrystalline cellulose was studied. Enzymes were illu- minated with WLPL for 60 min and 120 min and for each native and illuminated enzyme sample specific activity and kinetics of enzyme catalyzed hydrolysis of microcrystalline cellulose were established. Molecular weight Mw and radii if gyration Rg of protein chains of native and illuminated enzymes were measured by means of high pressure size exclu- sion chromatography coupled with multiangle laser light scattering and refractometric detectors (HPSEC-MALLS-RI). Conformations of protein chains of native and illuminated enzymes were evaluated on the basis of their circular dichro- ism (CD) spectra. Additionally, molecular weight M and radii of gyratio