卵巢颗粒细胞瘤中AMH表达及其组织学分级标准分析评价.docVIP

卵巢颗粒细胞瘤中AMH表达及其组织学分级标准分析评价[摘要] 目的:检测卵巢颗粒细胞瘤(granulosa cell tumor,GCT)组织中抗苗勒管激素(anti-mullerian hormone,AMH)的表达,并用它的表达水平及相关的统计学方法对卵巢GCT的组织学分级标准进行分析评价。方法:用免疫组织化学方法探讨AMH在40例卵巢GCT组织中的表达。结果:AMH在临床Ⅲ期病例中的阳性表达率明显低于其在临床Ⅰ期和Ⅱ期中的阳性表达率(P0.05)。李瑛氏组织学分级标准与临床分期有正相关关系(r=0.632,P0.05)。结论:李瑛氏组织学分级标准可用于病理学对卵巢GCT的预后评估。 [关键词] 卵巢肿瘤;颗粒细胞瘤;AMH;组织学分级 [中图分类号] R737.310.2 [文献标识码] C[文章编号] 1673-7210(2010)03(b)-059-03 Expression of AMH in ovarian granulosa cell tumor as well as the analysis and appreciation of its histological classification LIANG Xiaoyan1, YAO Hanwu1, MA Xiaoyu2 (1.Department of Pathology, the First Clinical Medical College of Shanxi Medical University, Taiyuan 030001, China; 2.The People’s Hospital of Pinglu County, Pinglu 044300, China) [Abstract] Objective: To detect the expression of AMH in ovarian granulosa cell tumor and to evaluate the criterion of histological classification with the expression of AMH and related statistical method. Methods: Expression of AMH was measured with immunohistochemistry in 40 cases of ovarian granulosa cell tumor. Results: The expression ratios of AMH in ovarian granulosa cell tumor decreased from stageⅠto stage Ⅲ, and the ratios of stageⅠand stage Ⅱ were significantly higher than those of stage Ⅲ (P0.05). The criterion of Li Ying-shi′s histological classification was positively correlated with clinical stage (r=0.632, P0.05). Conclusion: The criterion of Li Ying-shi′s histological classification can be used to evaluate the prognosis of ovarian GCT in pathology. [Key words] Ovarian tumor; Granulosa cell tumor; AMH; Histological classification 卵巢颗粒细胞瘤的组织学形态多种多样,且常混合存在,所以给组织学分级带来了一定的困难,目前尚无公认的统一标准[1]。肿瘤的组织学分级则是病理学上用于评价肿瘤恶性程度及预后的可靠的参考指标。Philip等[2]和李瑛氏[3]分别提出了针对卵巢GCT的组织学分级标准,而AMH则与卵巢的早期定向分化有关,并且是卵巢GCT敏感、特异和可靠的标志物之一[4]。本实验用AMH在卵巢GCT的表达水平和相关的统计学方法对两种组织学分级标准进行评价,以用于病理学对卵巢GCT的预后评估。 1 材料与方法 1.1 材料 40例卵巢GCT石蜡标本来源于山西医科大学第一临床医院1998～2009年的病理档案。卵巢GCT患者年龄15～70岁,平均45.5岁;50岁16例;病变均为单侧(左21例,右19例)。所有病例均有完整的临床病理资料。 1.2 方法 调取相应蜡