大孔树脂纯化盐地碱蓬红色素的工艺研究-广东农业科学.PDFVIP

东农业科学 2015 年第 4 期 67 大孔树脂纯化盐地碱蓬红色素的工艺研究 许 杰 滨州职业学院生物工程学院袁 山东 滨州 256603 冤 要 为纯化盐地碱蓬红色素袁提高其色价袁选取 X -5尧HPD300 尧D101尧HPD400尧NKA -9 和A B-8 等 6 种 大孔树脂对盐地碱蓬红色素进行静态吸附试验袁筛选最佳纯化色素树脂袁并研究最佳树脂对盐地碱蓬红色素 的纯化工艺条件遥结果表明袁X-5 大孔树脂对盐地碱蓬红色素的吸附和解吸效果较好遥在动态吸附中袁当上样液 度为 1 mg/mL尧pH 值为 6尧流速为 5 BV/h 时袁X-5 对盐地碱蓬红色素吸附效果最好曰以60% 乙醇溶液为洗脱 剂袁洗脱流速为 5 BV/h 时袁解吸效果最好曰盐地碱蓬红色素纯化后袁色价提高了6.14 倍遥 关键词 盐地碱蓬曰 红色素曰 大孔树脂曰 纯化 中图分类号 TS264.4 文献标识码 A 文章编号 1004-874X 2015冤04-0067-04 Study on purification of red pigment from Suaeda salsa by macroporous resin XU J ie (De partme nt of Bioenginee ring, Binzhou V ocational College , Binzhou 256603, China) A bstract: To elevate the red pigment from Suae da salsa color value, the characteristics of static adsorption and desorption of six kinds of macroporous resins (X -5, HPD300, D101, HPD400, NKA -9, and A B -8) to red pigment from S. salsa were investigated. T he purification processes conditions of the best macroporous resin for red pigment from S. salsa were also studied. T he result indicated that X -5 resin was suitable for purify ing red pigment from S. salsa. T he appropriate dynamic conditions were liquid concentration 1 mg/mL, pH value 6, and flow rate 5 BV/h. A nd using 60% ethanol as effluent at 5 BV/h in speed, after purification, the color value of purified pigment was 6.14 times higher than the original one. Key words: Suaeda salsa; red pigment; macro