PEG介导细胞融合最适条件探讨.docVIP

PEG介导细胞融合最适条件探讨 摘 要: 细胞融合是细胞工程中一项基本内容和基础技术,PEG是当今教学和科研中使用最多的一种化学诱导剂,以PEG为诱导剂,从不同浓度的PEG处理和不同时间的PEG处理两个方面来研究对鸡血红细胞融合的影响:鸡血红细胞融合率在PEG处理时间增加而提高;PEG浓度在30%~50%时,鸡血红细胞的融合率随PEG浓度升高而增加PEG浓度为50%时,鸡血红细胞的融合率最高,当PEG超过50%时,鸡血红细胞融合率下降,用浓度为50%的PEG处理鸡血红细胞20min,是鸡血红细胞的融合的最适条件:鸡血红细胞;细胞融合;PEG;融合率8137 Optional Conditions of PEG-Induced Cell Fusion Abstract: Cell fusion is a basic content and cell engineering technology, including PEG (polyethylene glycol) is a kind of chemical inducers, which is widely used in the modern teaching and research. This article mainly took chicken blood red blood cells for research materials, and PEG as inducers. In order to study the influence of PEG treatment on chicken red blood cell fusion, different PEG concentrations and different PEG-induced time was set in this experiment. The experimental results showed that the increase in chicken blood erythrocyte aggregation rate was observed with the increase in PEG treatment time, and that the increase in chicken blood red blood cell fusion rate was detected with increased concentrations of PEG, the concentration of which ranged from 30% to 50%. The best concentration of PEG (50%) facilitated the chicken blood red blood cell fusion. However, when the concentration was beyond 50%, chicken blood erythrocyte aggregation rate declined. In conclusion, the best concentration of PEG is 50%, and the best treatment time is 20 min for the chicken blood red blood cell fusion. 1 实验仪器?材料和试剂 1.1仪器 注射器5ml刻度离心管 1.2材料 市购家鸡 1.3试剂 1.3.1 0.85%生理盐水 称取NaCl 0.85g,溶于100ml的蒸馏水中 1.3.2 GKN液 称取NaCl 80.0g,葡萄糖2.0g,Na2HPO4bull;2H2O1.77g,KCl 0.4g,NaH2PO4bull;H2O 0.69g,酚红0.01g,溶于适量蒸馏水中,然后定容到1000ml 1.3.3 Hank原液(10times;) NaCl80.0g KCl4.0 g MgSO4bull;7H2O2.0 g Na2HPO4bull;12H2O1.2 g KH2PO40.6 g 葡萄糖10.0 g CaCl21.4 g 配置方法: (1) 先称取CaCl21.4g,溶于50ml的蒸馏水中 (2) 取1000ml的烧杯及容量瓶各一个,先放蒸馏水800ml于烧杯中,然后按上述配方顺序,逐一称取药品,才能加入下一样药品,直到葡萄糖完全溶解后,再将已溶解的CaCl2加入,最后加蒸馏水定容至1000ml PEG介导细胞融合最适条件探讨(2): 1 / 1