低聚果糖靶向调节肠道菌群缓解力竭运动导致的脂质-现代食品科技.pdf

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.5 低聚果糖靶向调节肠道菌群缓解力竭运动导致的 脂质代谢紊乱 张娜，李晶，宋佳，毛相朝，王玉明，王静凤，唐庆娟 （中国海洋大学食品科学与工程学院，山东青岛 266003) 摘要：研究低聚果糖对力竭运动所致脂质代谢紊乱的调节作用。采用 Balb/c 雄性小鼠，并将其随机分为 3 组，即正常组，力竭 运动组和低聚果糖干预组。本研究采用小鼠转轮式疲劳仪力竭运动模型，力竭运动组和低聚果糖组小鼠给予力竭运动干预，而正常组 保持静止。连续喂养 16 d 后，测定血清血脂指标并无菌收集小鼠粪便，进行肠道菌群的宏基因组分析。结果显示：力竭运动导致小 鼠脂质代谢紊乱，血清总胆固醇（TC ）、低密度脂蛋白（LDL-C ）和高密度脂蛋白（HDL-C ）水平显著升高（p 0.05 ），而甘油三酯 （TG ）水平显著降低（p 0.01 ）。同时，低聚果糖能显著改变力竭运动小鼠肠道菌群结构以及菌群功能。总之，低聚果糖可靶向调节 肠道菌群结构，改善肠道菌群脂质代谢通路，调控脂肪酸氧化和脂质合成相关酶，进而缓解力竭运动导致的脂质代谢紊乱。 关键词：低聚果糖；力竭运动；脂质代谢紊乱；肠道菌群；宏基因组 文章篇号：1673-9078(2017)5-7-13 DOI: 10.13982/j.mfst.1673-9078.2017.5.002 Fructo-oligosaccharides Protect Mice against Exhaustive Exercise-induced Dyslipidemia by Modulating Gut Microbial Composition and Function ZHANG Na, LI Jing, SONG Jia, MAO Xiang-zhao, WANG Yu-ming, WANG Jing-feng, TANG Qing-juan (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China) Abstract: To investigate the possible mechanism of fructo-oligosaccharides (FOS) in regulating lipid metabolism, male balb/c mice were randomly divided into three groups: control group (Control), exhaustive exercise group (Motion), and FOS intervention group (FOS). A forced exercise wheel-track treadmill was used in this study, and animals in both Motion and FOS groups were subjected to an exhaustive exercise protocol while the animals in the Control group were kept still. After sixteen days of treatments, the related serum lipid parameters were measured and fecal samples were collected to investigate gut microbiota changes by metagenome. The results showed