不同方法取材衣原体pcr检测结果比较分析（Comparative analysis of the results of Chlamydia trachomatis PCR test with different methods）.docVIP

不同方法取材衣原体pcr检测结果比较分析（Comparative analysis of the results of Chlamydia trachomatis PCR test with different methods） Blame gonococcus sex urethritis, it is to point to the urethritis that causes by the pathogen of other coccus outside gonococcus, this disease already exceeded clap in Euramerican country, and rank the first place of sexual transmission disease. Cases are also increasing in China, becoming one of the most common sexually transmitted diseases. Chlamydia trachomatis is one of the main pathogens of nongonococcal urethritis, and 40% ~ 50% of nongonococcal urethritis is caused by Chlamydia trachomatis [1]. The diagnosis of chlamydial infection is determined by etiological examination. In addition to the development of various detection techniques, many scholars will focus on the improvement of specimen selection. Based on the parasitic characteristics and pathogenicity of Chlamydia trachomatis, the effects of various sampling methods on the rate of physical examination of Chlamydia trachomatis were compared with those collected from Chlamydia trachomatis epithelial cells. The results showed that the urine samples were taken by urethral swab before urination in the morning, and the detection rate of Chlamydia in mixed specimens was the highest. It is reported as follows. 1 data and methods 1.1 test objects, Department of Dermatology, Huidong peoples Hospital, from May 2002 to November 2003, 392 cases of nongonococcal urethritis patients, aged 21~42 years, all male. All cases were randomly divided into 3 groups. 1.2 sampling method A group: swab and first times morning urine sampling, 89 cases. From morning urination, with as little as possible to avoid the cotton swab, the amount of urethral injury cases as little as possible, the depth of 2cm into the urethra, rotate gently swab 2 ~ 3 weeks, remove the swab, urinate, collecting forepart urine above 10ml in sterile test tube, centrifugation, precipitation with saline washed 3 time, precipitation and urethral