好好好细胞培养中的细节问题(排版)（Good good details in cell culture (typesetting)）.docVIP

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好好好细胞培养中的细节问题(排版)（Good good details in cell culture (typesetting)）.doc

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好好好细胞培养中的细节问题(排版)（Good good details in cell culture (typesetting)）

好好好细胞培养中的细节问题(排版)（Good good details in cell culture (typesetting)） Details in cell culture March 27, 2012 Basic techniques for aseptic manipulation 1. experiments were carried out before, the asepsis room and aseptic operation station (laminar flow) were irradiated with ultraviolet light for 30-60 minutes sterilization, with 70%ethanol clean aseptic operation bed, and open the aseptic fan running after 10 minutes, before the beginning of experiment operation. Only one cell line was treated at each time, and the medium was not shared even if the medium was the same to avoid mistakes, confusion or intercellular contamination. After the experiment, the experimental objects were removed from the worktable and cleaned with 70% ethanol for aseptic operation. The operating interval shall allow the sterile operating table to operate for more than 10 minutes before performing the operation of the next cell line. 2. aseptic work area should be kept clean and spacious, the necessary items, such as test tube rack, suction device or Straw Straw boxes can be temporarily placed in other experimental activities should be used to facilitate the circulation of air removal. The experimental articles were cleaned by 70% ethanol before they were brought into the aseptic operating table. The experimental operation shall be in the central sterile area of the lifting surface, not in the non sterile area of the edge. 3. be careful to use sterile lab articles to avoid contamination. Do not touch the tip of the straw or the mouth of the container. Do not operate on the container just above the opening. When the container is opened, hold the bottle cap with your hands and hold the bottle. Tilt about 45 degrees for use. Try not to place the top of the bottle cap up and place the table top. 4., the staff should pay attention to their own safety, and must wear experimental clothing and gloves before the experiment. For cell lines from humans or viruses, particular care should be taken and appropr