泌尿生殖道和呼吸道非淋球菌及非脑膜炎球菌奈瑟-第三军医大学学报.docVIP

贵州深部感染常见病原真菌的基因与研究 罗振华，王和*，王丹霓，康颖倩摘 要：目的 探讨贵州深部感染常见真菌的基因及其分布。方法采集贵州省不同地区242例深部感染标本分离真菌，用PCR扩增和DNA序列分析检测菌的rDNA的ITS1-5.8S rDNA-ITS2和25 rDNAⅠ型内含子序列。结果 242例深部感染患者标本共分离真菌261株，ITS序列含酵母菌246株（94.3%）和15株（5.7%）酵母菌白假丝酵母菌155株（59.3%）25S rDNAⅠ型内含子基因分别鉴定为A型83株（53.6%）、B型43株（27.7%）C型29株（18.7%）。结论 贵州地区深部感染的真菌酵母菌常见，其中主要是白假丝酵母菌及其基因A型。 ITS序列和25S rDNAⅠ型内含子序列的检测有助于真菌的快速诊断。广泛存在的不同种类酵母菌感染以及复数真菌感染，成为导致贵州地区深部真菌感染患者临床治疗困难的一个重要因素。 关键词：真菌；诊断；ITS；25S rDNA I intron Gene analysis and studies of the pathogenic fungus from the deep infection in Guizhou area Luo Zhen-hua, Wang He, Wang Dan-ni, Kang Ying-qian. Department of Microbiology, Guiyang Medical College, Guiyang, Guizhou（550004）,China Abstract: Objective genotype and distribution of the pathogenic fungus isolated from the patients with deep infectious diseases in Guizhou province. Methods The 242 samples were collected from the patients with the deep infectious diseases in the hospitals at different area in Guizhou. The ITS and 25S rDNA I intron sequences of the strains isolated were amplified and determined by the methods of the PCR and DNA 基金资助：贵阳市云岩区科技基金（云科技合同字[2008]第1-7号）和贵州省卫生厅科技基金（G2008-7）资助项目。 作者简介：罗振华,1977年生,女,讲师,从事微生物学与微生物感染的研究。E-mail:cwdbwh@ *通讯作者：王和，E-mail：cwdbwh@.sequencing. Results 261 strains of the fungi isolated from the 242 samples in which were including 246 yeast strains (94.3%) and 15 mold strains (5.7%) by the ITS sequences. By the genotype classification of the 25S rDNA I intron sequences, 155 strains of C. albicans were isolated and in which 83 strains belong to genotype A （53.6%）,43 strains are genotype B（27.7%）and 29 strains are genotype C（18.7%）.Conclusion Yeasts are the most common pathogenic fungi in the patients with deep fungus infection, in which there is a high percentage of C.albicans genotype A in Guizhou area. And the genetic analyses of ITS and 25S rDNA I intron sequences can be the useful molecular methods for the rapid diagnosis of fungal infections. The fungal infection with variable species and the mult