天然珙桐群体的rapd标记遗传多样性研究华中农业大学.pdfVIP

第 40 卷 第 4 期 林 业 科 学 Vol40 , No4 2 0 0 4 年 7 月 SCIENTIA SILVAE SINICAE Jul. , 2 0 0 4 天然珙桐群体的 RAPD 标记遗传多样性研究 宋丛文　包满珠 (华中农业大学　武汉 430070) 摘 　要 : 　利用 RAPD 技术 ,通过 13 个引物对 5 个天然珙桐种群的遗传多样性、种群内和种群间的遗传变异进行了 研究。结果表明 :珙桐天然种群具有丰富的遗传多样性 ,但群体间的差异明显 ,26 %的遗传变异存在于群体间 ,与 我国濒危树种马褂木和银杉等相似 ,而与广布性树种相异。取样方法对遗传多样性参数的影响分析表明 ,有效等 位基因数和基因多样度受取样个体数的影响较小 , 群体间的分化系数和基因流的估算受取样个体数的影响较大。 研究还将珙桐划分为东南部和西北部两大种源区。通过珙桐遗传多样性的研究 ,为今后有效保存和合理利用珙桐 种质资源提供了理论依据。 关键词 : 　珙桐 , 群体 , RAPD , 遗传多样性 ( ) 中图分类号:S71846 　　　文献标识码 :A 　　　文章编号 :1001 - 7488 2004 04 - 0075 - 05 Study on Genetic Diversity of RAPD Mark for Natural Davidia involucrata Population Song Congwen 　Bao Manzhu ( Huazhong Agricultural University 　Wuhan430070) Abstract : 　Davidia involucrata which belongs to Nyssaceae section monotype genus is peculiar plant in China , being old origin and survivor of tropical flora of Tertiary Period , it is classified as the firstgrade state protection plant. The genetic diversity and genetic variation within populations and interpopulations from 5 D . involucrata natural populations by 13 primers have been studied based on RAPD analysis. The results showed that D . involucrata natural population had rich genetic diversity , but the difference was significant among the populations , and 26 % of genetic variation existed among the populations , which was similar to endangered tree species of Liriodendron chinense and Cathaya argyrophylla in our country , but different from widelydi