20083067-程建宝-双抗水稻条纹病毒和水稻黑条矮缩病毒转基因水稻的培育.docVIP

双抗和转基因水稻的培育RNA介导的病毒抗性成为一个研究的热点，RNA介导的病毒抗性具有抗病性强（近似于免疫）、抗性持久、生物安全性高等特点。为克服RNA介导的病毒抗性的抗病谱较窄的特点，将产生抗性所需要的有效片段构建于同一质粒，导入植物以扩大抗性范围，获得的抗多种病毒的转基因植物。本研究以水稻条纹病毒Rice stripe virus，RSV）和黑条矮缩病毒(Rice black streaked dwarf virus， RBSDV为对象，用RSV和RBSDV的序列构建RNAi载体Dual anti-RSV and RBSDV the cultivation of genetically modified rice Author Cheng Jian-bao2008 Grade Bio-technology Major Adviser Song Yun-zhi Associate researcher Abstract: Rice stripe disease Rice and rice black streaked dwarf disease is a threat to rice production in both diseases. In recent years, RNA-mediated virus resistance has become a hot research, RNA-mediated virus resistance, strong resistance (similar to immunization), lasting resistance, bio-safe characteristics. In order to overcome the narrow spectrum of the characteristics of the disease resistance of the RNA-mediated virus resistance, will produce the resistance required for effective fragment is built on the same plasmid into plants in order to expand the resistance range, acquired resistance to a variety of viruses genetically modified plants. In this study, the rice stripe virus ( the stripe virus , RSV) and black-streaked dwarf virus (Rice black streaked dwarf virus, RBSDV) object, use RSV and RBSDV of CP gene sequences to construct the RNAi vector (MISK3), the use of Agrobacterium-mediated transformation method for anti-RSV and RBSDV transgenic rice. The present study, preliminary exploration for the cultivation of disease-resistant transgenic rice. Key words: rice stripe virus; rice black streaked dwarf virus; RNA interference; amiRNA; transgenic rice 1前言 水稻是我国最重要的粮食作物之一，在粮食生产和消费中占有主导地位。在过去30年间，水稻约占我国粮食种植面积的30%，占粮食总产量的40%左右，水稻病毒病曾给我国的水稻生产带来毁灭性损失。近年来，在我国粳稻种植区发生严重、对水稻生产造成很大威胁的水稻条纹叶枯病和水稻黑条矮缩病分别由水稻条纹病毒(Rice stripe virus， RSV)和水稻黑条矮缩病毒(Rice black streaked dwarf virus， RBSDV)引起。这两种病毒病的流行都与品种的抗病性、介体灰飞虱种群数量、带毒率及迁飞时期、耕作制度等密切相关。由于抗病品种在品质上的不足，导致感病品种大面积种植，再加上灰飞虱传毒的瞬时性和持久性，病毒病防治药剂的缺乏，使得治虫防病非常困难，最为经济有效的途径是利用品种自身的抗性达到主动防治病毒病害目的。然而传统的育种方法由于缺乏理想的抗源(国内尚未发现抗R