anti transglutaminase antibodies cause ataxia in mice反转谷氨酰胺酶抗体导致老鼠共济失调.pdfVIP

Anti Transglutaminase Antibodies Cause Ataxia in Mice 1 2 3 1 2 Sabrina Boscolo *, Andrea Lorenzon , Daniele Sblattero , Fiorella Florian , Marco Stebel , Roberto 1 4 5 4 6 Marzari , Tarcisio Not , Daniel Aeschlimann , Alessandro Ventura , Marios Hadjivassiliou , Enrico Tongiorgi1,7 1 Department of Life Sciences, University of Trieste, Trieste, Italy, 2 CSPA, University of Trieste, Trieste, Italy, 3 Department of Medical Science and IRCAD, University of Eastern Piedmont, Novara, Italy, 4 Institute for Childhood Disease, IRCCS Burlo Garofolo, Department of Reproductive and Development Science, University of Trieste, Trieste, Italy, 5 Matrix Biology and Tissue Repair Research Unit, School of Dentistry, Cardiff University, Cardiff, United Kingdom, 6 Department of Clinical Neurology, Royal Hallamshire Hospital, Sheffield, United Kingdom, 7 BRAIN Centre for Neuroscience, Department of Life Sciences, University of Trieste, Trieste, Italy Abstract Background: Celiac disease (CD) is an autoimmune gastrointestinal disorder characterized by the presence of anti- transglutaminase 2 (TG2) and anti-gliadin antibodies. Amongst the neurological dysfunctions associated with CD, ataxia represents the most common one. Methods: We analyzed by immunohistochemistry, the anti-neural reactivity of the serum from 20 CD patients. To determine the role of anti-TG2 antibodies in ataxia, two anti-TG2 single chain variable fragments (scFv), isolated from a phage-display IgA antibody library, were characterized by immunohistochemistry and ELISA, and injected in mice to study their effects on motor co