anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene抗血管生成和抗炎特性kahweol,咖啡二萜.pdfVIP

Anti-Angiogenic and Anti-Inflammatory Properties of Kahweol, a Coffee Diterpene ´ 1 1,2 1,2 Casimiro Cardenas , Ana R. Quesada , Miguel A. Medina * ´ ´ ´ 1 Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Malaga, Malaga, Spain, 2 CIBER de Enfermedades Raras (CIBERER), Malaga, Spain Abstract Background: Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecules. Methodology/Principal Findings: The experimental procedures included in vivo angiogenesis assays (both the chicken and quail choriallantoic membrane assay and the angiogenesis assay with fluorescent zebrafish), the ex vivo mouse aortic ring assay and the in vitro analysis of the effects of treatment of human endothelial cells with kahweol in cell growth, cell viability, cell migration and zymographic assays, as well as the tube formation assay on Matrigel. Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein-1. We show for the first time that kahweol is an anti- angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation,