antibodies elicited in response to ebna-1 may cross-react with dsdna抗体引起的反应与dsdna ebna-1可能交叉作用.pdfVIP

Antibodies Elicited in Response to EBNA-1 May Cross- React with dsDNA 1,2 3 3 2,3,4 4 3 Pragya Yadav , Hoa Tran , Roland Ebegbe , Paul Gottlieb , Hui Wei , Rita H. Lewis , Alice Mumbey- 4 4 4 2,3,4 Wafula , Atira Kaplan , Elina Kholdarova , Linda Spatz * 1 Department of Chemistry, The City College of New York and the Graduate Center of the City University of New York, New York, New York, United States of America, 2 The Ph.D. program in Biochemistry, The City College of New York and the Graduate Center of the City University of New York, New York, New York, United States of America, 3 The Graduate School of Biology, The City College of New York, New York, New York, United States of America, 4 Department of Microbiology and Immunology, Sophie Davis School of Biomedical Education, The City College of New York, New York, New York, United States of America Abstract Background: Several genetic and environmental factors have been linked to Systemic Lupus Erythematosus (SLE). One environmental trigger that has a strong association with SLE is the Epstein Barr Virus (EBV). Our laboratory previously demonstrated that BALB/c mice expressing the complete EBNA-1 protein can develop antibodies to double stranded DNA (dsDNA). The present study was undertaken to understand why anti-dsDNA antibodies arise during the immune response to EBNA-1. Methodology/Principal Findings: In this study, we demonstrated that mouse antibodies elicited in response to EBNA-1 cross-react with dsDNA. First, we showed that adsorption of sera reactive with EBNA-1 and dsDNA, on dsDNA cellulose columns, dim