anticonvulsive effect of paeoniflorin on experimental febrile seizures in immature rats possible application for febrile seizures in children抗惊厥的影响芍药苷在未成熟大鼠实验性发热性癫痫发作可能申请儿童发热性癫痫.pdfVIP

Anticonvulsive Effect of Paeoniflorin on Experimental Febrile Seizures in Immature Rats: Possible Application for Febrile Seizures in Children 1 2,3 1 2,3 1 1,3 Hitomi Hino , Hisaaki Takahashi , Yuka Suzuki , Junya Tanaka , Eiichi Ishii , Mitsumasa Fukuda * 1 Department of Pediatrics, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan, 2 Department of Molecular and Cellular Physiology, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan, 3 Ehime Proteo-Medicine Research Center, Department of Basic and Clinical Neuroscience, Ehime University, Toon, Ehime, Japan Abstract Febrile seizures (FS) is the most common convulsive disorder in children, but there have been no clinical and experimental studies of the possible treatment of FS with herbal medicines, which are widely used in Asian countries. Paeoniflorin (PF) is a major bioactive component of Radix Paeoniae alba, and PF-containing herbal medicines have been used for neuromuscular, neuropsychiatric, and neurodegenerative disorders. In this study, we analyzed the anticonvulsive effect of PF and Keishikashakuyaku-to (KS; a PF-containing herbal medicine) for hyperthermia-induced seizures in immature rats as a model of human FS. When immature (P5) male rats were administered PF or KS for 10 days, hyperthermia-induced seizures were significantly suppressed compared to control rats. In cultured hippocampal neurons, PF suppressed glutamate-induced elevation of intracellular Ca2+ ([Ca2+] ), glutamate receptor-mediated membrane depolarization, and glutamate-induced