barcoding and border biosecurity identifying cyprinid fishes in the aquarium trade条码和边境生物识别在水族馆贸易鲤科的鱼.pdfVIP

Barcoding and Border Biosecurity: Identifying Cyprinid Fishes in the Aquarium Trade 1 1 2 2 3 Rupert A. Collins *, Karen F. Armstrong , Rudolf Meier , Youguang Yi , Samuel D. J. Brown , Robert H. 3 4 4 Cruickshank , Suzanne Keeling , Colin Johnston 1 Bio-Protection Research Centre, Lincoln University, Canterbury, New Zealand, 2 Department of Biological Sciences and University Scholars Programme, National University of Singapore, Singapore, Singapore, 3 Department of Ecology, Faculty of Agriculture and Life Sciences, Lincoln University, Canterbury, New Zealand, 4 Animal Health Laboratory, Investigation and Diagnostic Centre, Ministry of Agriculture and Forestry, Upper Hutt, New Zealand Abstract Background: Poorly regulated international trade in ornamental fishes poses risks to both biodiversity and economic activity via invasive alien species and exotic pathogens. Border security officials need robust tools to confirm identifications, often requiring hard-to-obtain taxonomic literature and expertise. DNA barcoding offers a potentially attractive tool for quarantine inspection, but has yet to be scrutinised for aquarium fishes. Here, we present a barcoding approach for ornamental cyprinid fishes by: (1) expanding current barcode reference libraries; (2) assessing barcode congruence with morphological identifications under numerous scenarios (e.g. inclusion of GenBank data, presence of singleton species, choice of analytical method); and (3) providing supplementary information to identify difficult species. Methodology/Principal Findings: We sampled 172 ornamental cyprinid fish species from