bcl2a1a over-expression in murine hematopoietic stem and progenitor cells decreases apoptosis and results in hematopoietic transformationbcl2a1a表达小鼠造血干细胞和祖细胞的减少在造血细胞凋亡和结果转换.pdfVIP

BCL2A1a Over-Expression in Murine Hematopoietic Stem and Progenitor Cells Decreases Apoptosis and Results in Hematopoietic Transformation ´ 1. 1. 2 1,3 4 Jean-Yves Metais , Thomas Winkler , Julia T. Geyer , Rodrigo T. Calado , Peter D. Aplan , 5 1 Michael A. Eckhaus , Cynthia E. Dunbar * 1 Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Department of Pathology ˜ and Laboratory Medicine, Weill Cornell Medical College, New York, New York, United States of America, 3 Department of Internal Medicine, University of Sao Paulo at ˜ ˜ Ribeirao Preto Medical School, Ribeirao Preto, Brazil, 4 Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 5 Division of Veterinary Resources, National Institutes of Health, Bethesda, Maryland, United States of America Abstract We previously reported the development of a lethal myeloid sarcoma in a non-human primate model utilizing retroviral vectors to genetically modify hematopoietic stem and progenitor cells. This leukemia was characterized by insertion of the vector provirus into the BCL2A1 gene, with resultant BCL2A1 over-expression. There is little information on the role of this anti-apoptotic member of the BCL2 family in hematopoiesis or leukemia induction. Therefore we studied the impact of Bcl2a1a lentiviral over-expression on murine hematopoietic st