cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks – an mri studycardiosphere-derived梗塞的老鼠心脏细胞改善机能至少16周u2014u2014核磁共振研究.pdfVIP

Cardiosphere-Derived Cells Improve Function in the Infarcted Rat Heart for at Least 16 Weeks – an MRI Study 1 1,2 1,3 1 1 Carolyn A. Carr *, Daniel J. Stuckey , Jun Jie Tan , Suat Cheng Tan , Renata S. M. Gomes , Patrizia 1,2 4 4 5 1 Camelliti , Elisa Messina , Alessandro Giacomello , Georgina M. Ellison , Kieran Clarke 1 Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom, 2 National Heart and Lung Institute, Imperial College, London, United Kingdom, 3 Cluster of Cardiovascular Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang, Malaysia, 4 Department of Experimental Medicine, Cenci-Bolognetti Foundation, Pasteur Institute, University La Sapienza, Rome, Italy, 5 Stem Cell and Molecular Physiology Laboratory, Liverpool JM University, Liverpool, United Kingdom Abstract Aims: Endogenous cardiac progenitor cells, expanded from explants via cardiosphere formation, present a promising cell source to prevent heart failure following myocardial infarction. Here we used cine-magnetic resonance imaging (MRI) to track administered cardiosphere-derived cells (CDCs) and to measure changes in cardiac function over four months in the infarcted rat heart. Methods and Results: CDCs, cultured from neonatal rat heart, comprised a heterogeneous population including cells expressing the mesenchymal markers CD90 and CD105, the stem cell marker c-kit and the pluripotency markers Sox2, Oct3/ 4 and Klf-4. CDCs (2 6106) expressing green fluorescent protein (GFP+) were labelled with fluorescent micron-sized particles of iron oxide (MPI