brain-specific rescue of clock reveals system-driven transcriptional rhythms in peripheral tissuebrain-specific拯救时钟显示系统驱动转录节奏在外围组织.pdfVIP

Brain-Specific Rescue of Clock Reveals System-Driven Transcriptional Rhythms in Peripheral Tissue 1 2,3 2,3,4 2 2 Michael E. Hughes , Hee-Kyung Hong , Jason L. Chong , Alejandra A. Indacochea , Samuel S. Lee , 2,4 5,6 7 Michael Han , Joseph S. Takahashi *, John B. Hogenesch * 1 Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut, United States of America, 2 Center for Sleep and Circadian Biology, Northwestern University, Evanston, Illinois, United States of America, 3 Departments of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America, 4 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 5 Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 6 Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 7 Department of Pharmacology, Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America Abstract The circadian regulatory network is organized in a hierarchical fashion, with a central oscillator in the suprachiasmatic nuclei (SCN) orchestrating circadian oscillations in peripheral tissues. The nature of the relationship between central and peripheral oscillators, however, is poorly understood. We used the tetOFF expression system to specifically restore Clock function in the br