characterisation of human embryonic stem cells conditioning media by 1h-nuclear magnetic resonance spectroscopy描述的人类胚胎干细胞调节媒体1 h-nuclear磁共振光谱学.pdfVIP

Characterisation of Human Embryonic Stem Cells Conditioning Media by 1H-Nuclear Magnetic Resonance Spectroscopy 1¤a ´ ´ 2 ´ 1 ´ 2 David A. MacIntyre , Darıo Melguizo Sanchıs , Beatriz Jimenez , Ruben Moreno , Miodrag Stojkovic2¤b, Antonio Pineda-Lucena 1* ´ ´ ´ ´ 1 Structural Biochemistry Laboratory, Centro de Investigacion Prıncipe Felipe, Valencia, Spain, 2 Cellular Reprogramming Laboratory, Centro de Investigacion Prıncipe Felipe, Valencia, Spain Abstract Background: Cell culture media conditioned by human foreskin fibroblasts (HFFs) provide a complex supplement of protein and metabolic factors that support in vitro proliferation of human embryonic stem cells (hESCs). However, the conditioning process is variable with different media batches often exhibiting differing capacities to maintain hESCs in culture. While recent studies have examined the protein complement of conditioned culture media, detailed information regarding the metabolic component of this media is lacking. 1 1 Methodology/Principal Findings: Using a H-Nuclear Magnetic Resonance ( H-NMR) metabonomics approach, 32 metabolites and small compounds were identified and quantified in media conditioned by passage 11 HFFs (CMp11). A number of metabolites were secreted by HFFs with significantly higher concentration of lactate, alanine, and