characterization and whole genome analysis of human papillomavirus type 16 e1-1374∧63nt variants人类乳头瘤病毒类型的特征和全基因组分析16 e1 - 1374∧63 nt变体.pdfVIP

Characterization and Whole Genome Analysis of Human Papillomavirus Type 16 E1-1374 63nt Variants ˆ 1 2 3 4 Ivan Sabol , Mihaela Matovina , Ali Si-Mohamed , Magdalena Grce * 1 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia, 2 Department of Microbiology and Parasitology, School of Medicine, University of Rijeka, ˆ ´ Rijeka, Croatia, 3 Laboratoire de Virologie, Hopital Europeen Georges Pompidou, Paris, France, 4 Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia Abstract Background: The variation of the most common Human papillomavirus (HPV) type found in cervical cancer, the HPV16, has been extensively investigated in almost all viral genes. The E1 gene variation, however, has been rarely studied. The main objective of the present investigation was to analyze the variability of the E6 and E1 genes, focusing on the recently identified E1-1374 63nt variant. ˆ Methodology/Principal Findings: Variation within the E6 of 786 HPV16 positive cervical samples was analyzed using high- resolution melting, while the E1-1374 63nt duplication was assayed by PCR. Both techniques were supplemented with ˆ sequencing. The E1-1374 63nt duplication was linked with the E-G350 and the E-C109/G350 variants. In comparison to the ˆ referent HPV16, the E1-1374 63nt E-G350 variant was significantly associated with lower grade cervical lesions (p = 0.029), ˆ while the E1-1374 63nt E-C109/G350 varia