chronic rejection pathology after orthotopic lung transplantation in mice the development of a murine bos model and its drawbacks小鼠慢性排斥反应原位肺移植术后病理学的发展鼠bos模型及其缺陷.pdfVIP

Chronic Rejection Pathology after Orthotopic Lung Transplantation in Mice: The Development of a Murine BOS Model and Its Drawbacks ´ 1 6 2 1 Stephanie De Vleeschauwer , Wolfgang Jungraithmayr , Shana Wauters , Stijn Willems , Manuela 1 1 1 1 7 7 Rinaldi , Annemie Vaneylen , Stijn Verleden , Anna Willems-Widyastuti , Ken Bracke , Guy Brusselle , 5 2,4 1,3 1 Erik Verbeken , Dirk Van Raemdonck , Geert Verleden , Bart Vanaudenaerde * 1 Laboratory of Pneumology, Katholieke Universiteit Leuven, Leuven, Belgium, 2 Laboratory of Experimental Thoracic Surgery, Katholieke Universiteit Leuven, Leuven, Belgium, 3 Department of Pneumology, University Hospitals Leuven, Leuven, Belgium, 4 Department of Thoracic Surgery, University Hospitals Leuven, Leuven, Belgium, 5 Department of Pathology, University Hospitals Leuven, Leuven, Belgium, 6 Laboratory of Thoracic Surgery, University Hospital, Zurich, Switzerland, 7 Department of Respiratory Medicine, University Hospital Gent, Gent, Belgium Abstract Almost all animal models for chronic rejection (CR) after lung transplantation (LTx) fail to resemble the human situation. It was our attempt to develop a representative model of CR in mice. Orthotopic LTx was performed in allografts receiving daily immunosuppression with steroids and cyclosporine. Controls included isografts and mice only undergoing thoracotomy (SHAM). Allografts were sacrificed 2, 4, 6, 8, 10 or 12 weeks after LTx. Pulmonary function was measured repeatedly in the 12w allo