ciliary neurotrophic factor induces genes associated with inflammation and gliosis in the retina a gene profiling study of flow-sorted, müller cells睫状神经营养因子诱发与炎症和神经胶质过多症相关的基因在视网膜基因分析研究flow-sorted,muller细胞.pdfVIP

Ciliary Neurotrophic Factor Induces Genes Associated with Inflammation and Gliosis in the Retina: A Gene ¨ Profiling Study of Flow-Sorted, Muller Cells 1. 2. 1 2 2 1 Wei Xue , Radu I. Cojocaru , V. Joseph Dudley , Matthew Brooks , Anand Swaroop , Vijay P. Sarthy * 1 Department of Ophthalmology, Northwestern University Feinberg Medical School, Chicago, Illinois, United States of America, 2 Neurobiology, Neurodegeneration and Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States of America Abstract Background: Ciliary neurotrophic factor (CNTF), a member of the interleukin-6 cytokine family, has been implicated in the development, differentiation and survival of retinal neurons. The mechanisms of CNTF action as well as its cellular targets in the retina are poorly understood. It has been postulated that some of the biological effects of CNTF are mediated through its action via retinal glial cells; however, molecular changes in retinal glia induced by CNTF have not been elucidated. We ¨ have, therefore, examined gene expression dynamics of purified Muller (glial) cells exposed to CNTF in vivo. ¨ Methodology/Principal Findings: Muller cells were flow-sorted from mgfap-egfp transgenic mice one or three days after ¨ intravitreal injection of CNTF. Microarray analysis using RNA from purified Muller cells showed differenti