changes in human fecal microbiota due to chemotherapy analyzed by taqman-pcr, 454 sequencing and pcr-dgge fingerprinting人类粪便微生物群的变化由于化疗分析taqman-pcr,454测序和pcr-dgge指纹.pdfVIP

Changes in Human Fecal Microbiota Due to Chemotherapy Analyzed by TaqMan-PCR, 454 Sequencing and PCR-DGGE Fingerprinting 1 1 1 1 1 Jutta Zwielehner , Cornelia Lassl , Berit Hippe , Angelika Pointner , Olivier J. Switzeny , Marlene 1 2 2 1 Remely , Elvira Kitzweger , Reinhard Ruckser , Alexander G. Haslberger * 1 Department of Nutritional Sciences, Vienna, Austria, 2 Sozialmedizinisches Zentrum Ost, Vienna, Austria Abstract Background: We investigated whether chemotherapy with the presence or absence of antibiotics against different kinds of cancer changed the gastrointestinal microbiota. Methodology/Principal Findings: Feces of 17 ambulant patients receiving chemotherapy with or without concomitant antibiotics were analyzed before and after the chemotherapy cycle at four time points in comparison to 17 gender-, age- and lifestyle-matched healthy controls. We targeted 16S rRNA genes of all bacteria, Bacteroides, bifidobacteria, Clostridium cluster IV and XIVa as well as C. difficile with TaqMan qPCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting and high-throughput sequencing. After a significant drop in the abundance of microbiota (p = 0.037) following a single treatment the microbiota recovered within a few days. The chemotherapeutical treatment marginally affected the Bacteroides while the Clostridium cluster IV and XIVa were significantly more sensitive to chemotherapy and antibiotic treatment. DGGE fingerprinting showed decreased diversity of Clostridium cluster IV and XIVa in response to chemotherapy with cluster IV diversity being p