comparative expression profile of mirna and mrna in primary peripheral blood mononuclear cells infected with human immunodeficiency virus (hiv-1)比较的microrna的表达谱和mrna在初级外周血单核细胞感染人类免疫缺陷病毒(hiv - 1).pdfVIP

Comparative Expression Profile of miRNA and mRNA in Primary Peripheral Blood Mononuclear Cells Infected with Human Immunodeficiency Virus (HIV-1) 1. 1. 2 1 1 Ankit Gupta , Pruthvi Nagilla , Hai-Son Le , Coulton Bunney , Courtney Zych , Anbupalam 3 2 3 1 Thalamuthu , Ziv Bar-Joseph , Sinnakaruppan Mathavan , Velpandi Ayyavoo * 1 Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 2 Department of Machine Learning, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America, 3 Human Genetics, Genome Institute of Singapore, Singapore Abstract Host cells respond to exogenous infectious agents such as viruses, including HIV-1. Studies have evaluated the changes associated with virus infection at the transcriptional and translational levels of the cellular genes involved in specific pathways. While this approach is useful, in our view it provides only a partial view of genome-wide changes. Recently, technological advances in the expression profiling at the microRNA (miRNA) and mRNA levels have made it possible to evaluate the changes in the components of multiple pathways. To understand the role of miRNA and its interplay with host cellular gene expression (mRNA) during HIV-1 infection, we performed a comparative global miRNA and mRNA microarray using human PBMCs infected with HIV-1. The PBMCs were derived from multiple donors and were infected with virus generated from the molecular clone pNL4-3. The results showed that HIV-1 infection led to altered regulation of