circadian rhythm and cartilage extracellular matrix genes in osseointegration a genome-wide screening of implant failure by vitamin d deficiency昼夜节律和软骨细胞外基质基因在骨整合的全基因组筛查植入失败由于缺乏维生素d.pdfVIP

Circadian Rhythm and Cartilage Extracellular Matrix Genes in Osseointegration: A Genome-Wide Screening of Implant Failure by Vitamin D Deficiency 1,2 1,3 1 4 Cristiane Machado Mengatto , Federico Mussano , Yoshitomo Honda , Christopher S. Colwell , Ichiro Nishimura1* 1 Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, University of California Los Angeles School of Dentistry, Los Angeles, California, United States of America, 2 Department of Prosthodontics and Periodontology, Piracicaba School of Dentistry, State University of Campinas, Piracicaba, Sao Paulo, Brazil, 3 Department of Biomedical Science and Human Oncology, University of Turin, Turin, Italy, 4 Department of Psychiatry and Biobehavioral Science, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, United States of America Abstract Background: Successful dental and orthopedic implants require the establishment of an intimate association with bone tissue; however, the mechanistic explanation of how biological systems accomplish osseointegration is still incomplete. We sought to identify critical gene networks involved in osseointegration by exploring the implant failure model under vitamin D deficiency. Methodology: Adult male Sprague-Dawley rats were exposed to control or vitamin D-deficient diet prior to the osteotomy surgery in the femur bone and the placement of T-shaped Ti4Al6V implant. Two weeks after the osteotomy and implant placement, tissue formed at the osteotomy site or in the hollow chamber of T-shaped implant was harvested and total RNA was evaluated by whole genome microa