changes in the relative inflammatory responses in sheep cells overexpressing of toll-like receptor 4 when stimulated with lps的变化相对羊细胞炎症反应overexpressing toll样受体4与lps刺激时.pdfVIP

Changes in the Relative Inflammatory Responses in Sheep Cells Overexpressing of Toll-Like Receptor 4 When Stimulated with LPS 1. 2. 1 5 3 1 6 Shoulong Deng , Qian Wu , Kun Yu , Yunhai Zhang , Yuchang Yao , Wenting Li , Zhuo Deng , 1 3 1,4 Guoshi Liu , Wu Li , Zhengxing Lian * 1 Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beij ing, P.R. China, 2 School of Biological Science and Medical Engineering, Beij ing University of Aeronautics and Astronautics, Beij ing, P.R. China, 3 College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China, 4 National Key Lab of Agrobiotechnology, Beij ing, P.R. China, 5 College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China, 6 Department of Animal Science, Oklahoma State University, Stillwater, Oklahoma, United States of America Abstract Background: Many groups of Gram-negative bacteria cause diseases harmful to sheep. TLR4 is an important Toll-like receptor (TLR) which responds to common Gram-negative bacterial infections. Activation of TLR4 leads to the induction of inflammatory responses, which is a linkage between the innate and adaptive immune systems. A vector pTLR4-3S was constructed to overexpress TLR4 gene in sheep. In this study, effects of TLR4 overexpression on inflammation response under LPS stimulated were addressed in vivo and in vitro. Methodology/Principal Findings: Sheep fetal fibroblasts were transfected with expression vector pTLR4-3S. Transgenic sheep were produced by microinjection of the